ELISA/assay

Mouse Bone Morphogenetic Protein 4 ELISA Kit

MBS732742

48-Strip-Wells

470 USD

ELISA Kit

Mouse Bone Morphogenetic Protein 4 ELISA Kit

Bone Morphogenetic Protein 4

BMP4; BMP4; bone morphogenetic protein 4; etID309887.17; bone morphogenetic protein 4; BMP4

BMP4

bmp4; bmp4; bmp-4; zbmp-4; zgc:100779; BMP4

O13107_DANRE

Mouse

This assay has high sensitivity and excellent specificity for detection of BMP4. No significant cross-reactivity or interference between BMP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BMP4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This BMP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BMP4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: BMP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMP4 antibody and an BMP4-HRP conjugate. The assay sample and buffer are incubated together with BMP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP4 concentration since BMP4 from samples and BMP4-HRP conjugate compete for the anti-BMP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP4 from the sample, fewer sites are left to bind BMP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP4 concentration in each sample is interpolated from this standard curve.

2149144

AAC60285.1

45,758 Da

Adipogenesis Pathway (921024); BMP Signaling Pathway (198931); FGF Signaling Pathway (198948); Hedgehog Signaling Pathway (83640); Hedgehog Signaling Pathway (474); TGF-beta Signaling Pathway (83641); TGF-beta Signaling Pathway (475); Canonical Wnt - Zebrafish Pathway (198928); Noncanonical Wnt Pathway (198976)

48-Strip-Wells

470 USD

96-Strip-Wells

675