product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat 3-Nitrotyrosine ELISA Kit
catalog :
MBS732683
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
product information
catalog number :
MBS732683
products type :
ELISA Kit
products full name :
Rat 3-Nitrotyrosine ELISA Kit
products short name :
3-Nitrotyrosine
products gene name :
3-NT
reactivity :
Rat
specificity :
This assay has high sensitivity and excellent specificity for detection of 3NT. No significant cross-reactivity or interference between 3NT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 3NT and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0pg/mL.
products description :
Intended Uses This 3NT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat 3NT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: 3NT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for 3NT. Standards or samples are then added to the microtiter plate wells and 3NT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of 3NT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for 3NT are added to each well to "sandwichâ the 3NT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain 3NT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 3NT concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!