Human Elastin ELISA Kit | MyBioSource MBS732663 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Elastin ELISA Kit

catalog :

MBS732663

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS732663

products type :

ELISA Kit

products full name :

Human Elastin ELISA Kit

products short name :

Elastin

other names :

elastin; Elastin; elastin; tropoelastin; elastin; Tropoelastin

products gene name :

ELN

other gene names :

ELN; ELN; WS; WBS; SVAS

uniprot entry name :

ELN_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of ELN. No significant cross-reactivity or interference between ELN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ELN and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This ELN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ELN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cell Biology

products description :

Principle of the assay: ELN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ELN antibody and an ELN-HRP conjugate. The assay sample and buffer are incubated together with ELN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ELN concentration since ELN from samples and ELN-HRP conjugate compete for the anti-ELN antibody binding site. Since the number of sites is limited, as more sites are occupied by ELN from the sample, fewer sites are left to bind ELN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ELN concentration in each sample is interpolated from this standard curve.

ncbi gi num :

182021

ncbi acc num :

AAC98395.1

ncbi mol weight :

60,980 Da

ncbi pathways :

Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

ncbi summary :

This gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

elastin: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. Defects in ELN are the cause of cutis laxa, autosomal dominant, type 1 (ADCL1). A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema. Defects in ELN are the cause of supravalvular aortic stenosis (SVAS). SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome. ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. Belongs to the elastin family. 13 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: proteinaceous extracellular matrix; extracellular region. Molecular Function: protein binding; extracellular matrix structural constituent. Biological Process: extracellular matrix disassembly; cell proliferation; extracellular matrix organization and biogenesis; organ morphogenesis; blood circulation; respiratory gaseous exchange. Disease: Cutis Laxa, Autosomal Dominant 1; Williams-beuren Syndrome; Supravalvular Aortic Stenosis

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage