catalog number :
MBS732631
products type :
ELISA Kit
products full name :
Mouse Neurokinin A ELISA Kit
products short name :
Neurokinin A
other names :
neurokinin A
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
other info2 :
Intended Uses: This NKA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse NKA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Neurobiology
products description :
Principle of the Assay: NKA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NKA antibody and an NKA-HRP conjugate. The assay sample and buffer are incubated together with NKA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NKA concentration since NKA from samples and NKA-HRP conjugate compete for the anti-NKA antibody binding site. Since the number of sites is limited, as more sites are occupied by NKA from the sample, fewer sites are left to bind NKA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NKA concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAB24862.1