Human Annexin A2 ELISA Kit | MyBioSource MBS732593 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Annexin A2 ELISA Kit

catalog :

MBS732593

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS732593

products type :

ELISA Kit

products full name :

Human Annexin A2 ELISA Kit

products short name :

Annexin A2

other names :

annexin A2 isoform 2; Annexin A2; annexin A2; annexin-2; protein I; annexin II; lipocortin II; chromobindin 8; chromobindin-8; calpactin I heavy chain; calpactin-1 heavy chain; calpactin I heavy polypeptide; placental anticoagulant protein IV; epididymis secretory protein Li 270; annexin A2; Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; Chromobindin-8; Lipocortin II; Placental anticoagulant protein IV; PAP-IV; Protein I; p36

products gene name :

ANXA2

other gene names :

ANXA2; ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270; ANX2; ANX2L4; CAL1H; LPC2D; PAP-IV

uniprot entry name :

ANXA2_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of ANXA2. No significant cross-reactivity or interference between ANXA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANXA2 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

products categories :

Cell Biology

products description :

Intended Uses This ANXA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANXA2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ANXA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANXA2 antibody and an ANXA2-HRP conjugate. The assay sample and buffer are incubated together with ANXA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA2 concentration since ANXA2 from samples and ANXA2-HRP conjugate compete for the anti-ANXA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA2 from the sample, fewer sites are left to bind ANXA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA2 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

209862831

ncbi acc num :

NP_001129487.1

ncbi gb acc num :

NM_001136015.2

ncbi mol weight :

40,411 Da

ncbi pathways :

Dissolution Of Fibrin Clot Pathway (106061); Hemostasis Pathway (106028); Prostaglandin Synthesis And Regulation Pathway (198912)

ncbi summary :

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ANXA2: a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. Heterotetramer containing 2 light chains of S100A10 2 heavy chains of ANXA2. May cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion. Protein type: Calcium-binding; Motility/polarity/chemotaxis; Lipid-binding. Chromosomal Location of Human Ortholog: 15q22.2. Cellular Component: extrinsic to plasma membrane; extracellular space; protein complex; cell surface; lysosomal membrane; late endosome membrane; early endosome; lipid particle; cell cortex; lipid raft; extracellular matrix; ruffle; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; basement membrane; midbody; nucleus; endosome; sarcolemma; vesicle. Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; cytoskeletal protein binding; calcium ion binding; calcium-dependent protein binding; Rab GTPase binding. Biological Process: positive regulation of fibroblast proliferation; fibrinolysis; collagen fibril organization; negative regulation of catalytic activity; protein heterotetramerization; positive regulation of vesicle fusion; angiogenesis; positive regulation of binding; positive regulation of protein amino acid phosphorylation; body fluid secretion; lipid raft formation; membrane budding

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage