ELISA/assay

Human Urotensin II ELISA Kit

MBS732588

48-Strip-Wells

470 USD

ELISA Kit

Human Urotensin II ELISA Kit

Urotensin II

urotensin-2; Urotensin-2; urotensin-2; UII; U-II; urocortin 2; urotensin II; urotensin-II; urotensin 2; Urotensin II; U-II; UII

U II

Uts2; Uts2; Ucn2; U-II; UII

UTS2_RAT

Human

This assay has high sensitivity and excellent specificity for detection of U2. No significant cross-reactivity or interference between U2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between U2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This U2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human U2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay U2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-U2 antibody and an U2-HRP conjugate. The assay sample and buffer are incubated together with U2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the U2 concentration since U2 from samples and U2-HRP conjugate compete for the anti-U2 antibody binding site. Since the number of sites is limited, as more sites are occupied by U2 from the sample, fewer sites are left to bind U2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The U2 concentration in each sample is interpolated from this standard curve.

9507227

NP_062033.1

13,614 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1010164); G Alpha (q) Signalling Events Pathway (1010197); GPCR Downstream Signaling Pathway (1010193); GPCR Ligand Binding Pathway (1010163); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1010213); Peptide Ligand-binding Receptors Pathway (1010165); Signal Transduction Pathway (1010015); Signaling By GPCR Pathway (1010162)

a cyclic neuropeptide; may be involved in the control of neuromuscular functions [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675