Rat Proteolipid Protein 1, Myelin ELISA Kit | MyBioSource MBS732565 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Proteolipid Protein 1, Myelin ELISA Kit

catalog :

MBS732565

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS732565

products type :

ELISA Kit

products full name :

Rat Proteolipid Protein 1, Myelin ELISA Kit

products short name :

Proteolipid Protein 1, Myelin

other names :

PLP1; Myelin proteolipid protein; myelin proteolipid protein; lipophilin; major myelin proteolipid protein; proteolipid protein 1; Lipophilin

products gene name :

PLP1

other gene names :

PLP1; PLP1; PLP; PMD; HLD1; MMPL; SPG2; GPM6C; PLP/DM20; PLP; PLP

uniprot entry name :

MYPR_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of PLP. No significant cross-reactivity or interference between PLP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PLP and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This PLP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PLP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the assay: PLP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PLP. Standards or samples are then added to the microtiter plate wells and PLP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PLP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PLP are added to each well to “sandwich” the PLP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PLP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLP concentration in each sample is interpolated from this standard curve.

ncbi gi num :

49168568

ncbi acc num :

CAG38779.1

uniprot acc num :

P60201

ncbi mol weight :

26,274 Da

ncbi pathways :

Glial Cell Differentiation Pathway (698758); SIDS Susceptibility Pathways (198901)

ncbi summary :

This gene encodes a transmembrane proteolipid protein that is the predominant myelin protein present in the central nervous system. It may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause X-linked Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively spliced transcript variants encoding distinct isoforms or having different 5' UTRs, have been identified for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

PLP1: This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1); also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay. Defects in PLP1 are the cause of spastic paraplegia X- linked type 2 (SPG2). SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. Belongs to the myelin proteolipid protein family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell surface. Chromosomal Location of Human Ortholog: Xq22. Cellular Component: integral to membrane; plasma membrane; myelin sheath. Molecular Function: structural constituent of myelin sheath; structural molecule activity. Biological Process: integrin-mediated signaling pathway; synaptic transmission; substantia nigra development; myelination in the central nervous system; cell maturation; long-chain fatty acid biosynthetic process; axon ensheathment; inflammatory response; astrocyte development. Disease: Spastic Paraplegia 2, X-linked; Pelizaeus-merzbacher Disease

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage