ELISA/assay

Mouse Pulmonary surfatcant-associated protein A, SP-A ELISA Kit

MBS732517

48-Strip-Wells

470 USD

ELISA Kit

Mouse Pulmonary surfatcant-associated protein A, SP-A ELISA Kit

Pulmonary surfatcant-associated protein A, SP-A

Mouse Pulmonary surfatcant-associated protein A (SP-A) ELISA Kit; Mouse Pulmonary surfatcant-associated protein A, SP-A ELISA Kit; Pulmonary surfatcant-associated protein A, SP-A; Pulmonary surfatcant-associated protein A, SP-A ELISA Kit (Mouse)

SP-A; Pulmonary surfactant-associated protein A; pulmonary surfactant-associated protein A; PSAP; PSP-A; surfactant pulmonary associated protein A1; surfactant associated protein A1

SP-A

Sftpa1; Sftpa1; SP-A; Sftp1; Sftp-1; Sftp-1; Sftp1; Sftpa

SFTPA_MOUSE

Mouse

This assay has high sensitivity and excellent specificity for detection of SPA. No significant cross-reactivity or interference between SPA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SPA and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This SPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SPA. This ELISA kit for research use only, not for therapeutic or diagnostic applications

Mouse ELISA Kit

Principle of the assay: SPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SPA antibody and an SPA-HRP conjugate. The assay sample and buffer are incubated together with SPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPA concentration since SPA from samples and SPA-HRP conjugate compete for the anti-SPA antibody binding site. Since the number of sites is limited, as more sites are occupied by SPA from the sample, fewer sites are left to bind SPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPA concentration in each sample is interpolated from this standard curve.

260453

P35242

26,157 Da

Pertussis Pathway (218112); Pertussis Pathway (218099); Phagosome Pathway (153914); Phagosome Pathway (153859)

SFTPA2: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Defects in SFTPA2 are a cause of pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. Belongs to the SFTPA family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: multivesicular body; extracellular space; rough endoplasmic reticulum; cytoplasmic vesicle. Biological Process: positive regulation of phagocytosis

48-Strip-Wells

470 USD

96-Strip-Wells

675