ELISA/assay

Human Synaptosome Associated Protein 25 ELISA Kit

MBS732443

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Synaptosome Associated Protein 25 ELISA Kit

Synaptosome Associated Protein 25

SNAP-25; SNAP-25

SNAP 25

LOC100533400

Human

This assay has high sensitivity and excellent specificity for detection of SNAP-25. No significant cross-reactivity or interference between SNAP-25 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SNAP-25 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This SNAP-25 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SNAP-25. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: SNAP-25 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SNAP-25 antibody and an SNAP-25-HRP conjugate. The assay sample and buffer are incubated together with SNAP-25-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SNAP-25 concentration since SNAP-25 from samples and SNAP-25-HRP conjugate compete for the anti-SNAP-25 antibody binding site. Since the number of sites is limited, as more sites are occupied by SNAP-25 from the sample, fewer sites are left to bind SNAP-25-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNAP-25 concentration in each sample is interpolated from this standard curve.

325296753

NP_001191612.1

NM_001204683.1

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675