ELISA/assay

Human antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit

MBS732403

96 Strip Wells

675 USD

ELISA Kit

Human antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit

diuretic hormone/vasopressin/arginine vasopressin

Human antidiuretic hormone/vasopressin/arginine vasopressin ELISA Kit; antidiuretic hormone/vasopressin/arginine vasopressin; antidiuretic hormone/vasopressin/arginine vasopressin (Human)

Arginine vasopressin; Vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin 2-copeptin; neurohypophyseal; prepro-AVP-NP II; antidiuretic hormone; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II; arginine vasopressin; AVP-NPIICleaved into the following 3 chains:Arg-vasopressin; Alternative name(s):; Arginine-vasopressinNeurophysin 2; Alternative name(s):; Neurophysin-II

ADH/VP/AVP

AVP; AVP; VP; ADH; ARVP; AVRP; AVP-NPII; ARVP; VP

NEU2_HUMAN

Human

Store all reagents at 2-8 degree C

Human ELISA Kit

Intended Uses: This ADH/VP/AVP ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ADH/VP/AVP in the sample, this ADH/VP/AVP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ADH/VP/AVP concentration. The concentration of ADH/VP/AVP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This ADH/VP/AVP enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for ADH/VP/AVP. Standards or samples are then added to the microtiter plate wells and ADH/VP/AVP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADH/VP/AVP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADH/VP/AVP are added to each well to "sandwich" the ADH/VP/AVP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain ADH/VP/AVP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

116497129

AAI26197.1

P01185

17,325 Da

Aquaporin-mediated Transport Pathway 187187!!BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway 477138!!Circadian Clock Pathway 187173!!Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!G Alpha (q) Signalling Events Pathway 106043!!G Alpha (s) Signalling Events Pathway 119549!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 645295!!Peptide Ligand-binding Receptors Pathway 106358

This gene encodes a precursor protein consisting of arginine vasopressin and two associated proteins, neurophysin 2 and a glycopeptide, copeptin. Arginine vasopressin is a posterior pituitary hormone which is synthesized in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. Along with its carrier protein, neurophysin 2, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis where it is either stored or secreted into the bloodstream. The precursor is thought to be activated while it is being transported along the axon to the posterior pituitary. Arginine vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney, and also causes vasoconstriction of the peripheral vessels. This hormone can contract smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in this gene cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI). [provided by RefSeq, Mar 2010]

Function: Neurophysin 2 specifically binds vasopressin.Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Subcellular location: Secreted. Involvement in disease: Diabetes insipidus, neurohypophyseal (NDI) [MIM:125700]: A disease characterized by persistent thirst, polydipsia and polyuria. Affected individuals are apparently normal at birth, but characteristically develop symptoms of vasopressin deficiency during childhood.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.36. Sequence similarities: Belongs to the vasopressin/oxytocin family.

96 Strip Wells

675 USD