ELISA/assay

Rabbit Matrix metalloproteinase 2 ELISA Kit

MBS732397

48-Strip-Wells

470 USD

ELISA Kit

Rabbit Matrix metalloproteinase 2 ELISA Kit

Matrix metalloproteinase 2

MMP-2, partial; 72 kDa type IV collagenase; 72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; matrix metalloproteinase 2; matrix metalloproteinase-2; matrix metallopeptidase 2; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2

MMP-2

Mmp2; Mmp2; MMP-2

MMP2_RAT

Rabbit

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This MMP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit MMP-2. This ELISA kit for research use only, not for therapeutic or diagnostic applications

Cancer

Principle of the Assay: MMP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP-2 antibody and an MMP-2-HRP conjugate. The assay sample and buffer are incubated together with MMP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-2 concentration since MMP-2 from samples and MMP-2-HRP conjugate compete for the anti-MMP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-2 from the sample, fewer sites are left to bind MMP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-2 concentration in each sample is interpolated from this standard curve.

8050250

AAF71618.1

74,149 Da

Bladder Cancer Pathway (83503); Bladder Cancer Pathway (527); Estrogen Signaling Pathway (799190); Estrogen Signaling Pathway (799197); GnRH Signaling Pathway (83483); GnRH Signaling Pathway (502); IL-3 Signaling Pathway (198504); Leukocyte Transendothelial Migration Pathway (83475); Leukocyte Transendothelial Migration Pathway (494); Matrix Metalloproteinases Pathway (198492)

plays important roles in wound healing, angiogenesis, platelet aggregation, and tumor metastasis; mediates the response of the intrinsic glomerular mesangial cell to inflammatory stimuli [RGD, Feb 2006]

48-Strip-Wells

470 USD

96-Strip-Wells

675