catalog number :
MBS732373
products type :
ELISA Kit
products full name :
Mouse Allopregnanolone/3a, 5a-tetrahydroprogesterone ELISA Kit
products short name :
Allopregnanolone/3a, 5a-tetrahydroprogesterone
products gene name :
AP/3a-5a-THP
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This AP/3a-5a-THP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AP/3a-5a-THP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the Assay: AP/3a-5a-THP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AP/3a-5a-THP antibody and an AP/3a-5a-THP-HRP conjugate. The assay sample and buffer are incubated together with AP/3a-5a-THP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AP/3a-5a-THP concentration since AP/3a-5a-THP from samples and AP/3a-5a-THP-HRP conjugate compete for the anti-AP/3a-5a-THP antibody binding site. Since the number of sites is limited, as more sites are occupied by AP/3a-5a-THP from the sample, fewer sites are left to bind AP/3a-5a-THP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AP/3a-5a-THP concentration in each sample is interpolated from this standard curve.