ELISA/assay

Rat Pleiotrophin ELISA Kit

MBS732362

48-Strip-Wells

470 USD

ELISA Kit

Rat Pleiotrophin ELISA Kit

Pleiotrophin

PTN, partial; Pleiotrophin; Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Heparin-binding growth-associated molecule; HB-GAM; Heparin-binding neurite outgrowth-promoting factor 1; HBNF-1; Osteoblast-specific factor 1; OSF-1

PTN

PTN; HBNF1; NEGF1; PTN; HBBM; HBGF-8; HB-GAM; HBNF-1; OSF-1

PTN_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of PTN. No significant cross-reactivity or interference between PTN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTN and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This PTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PTN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the assay: PTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTN antibody and an PTN-HRP conjugate. The assay sample and buffer are incubated together with PTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTN concentration since PTN from samples and PTN-HRP conjugate compete for the anti-PTN antibody binding site. Since the number of sites is limited, as more sites are occupied by PTN from the sample, fewer sites are left to bind PTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTN concentration in each sample is interpolated from this standard curve.

47496623

CAG29334.1

P21246

18,942 Da

PTN: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti- apoptotic signaling of PTN and regulation of cell proliferation. Belongs to the pleiotrophin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q33. Cellular Component: extracellular space; endoplasmic reticulum. Molecular Function: heparin binding; growth factor activity; protein phosphatase inhibitor activity. Biological Process: nervous system development; positive regulation of cell division; positive regulation of cell proliferation; negative regulation of catalytic activity; learning; transmembrane receptor protein tyrosine phosphatase signaling pathway; bone mineralization

48-Strip-Wells

470 USD

96-Strip-Wells

675