product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Peroxisome Proliferators Activated Receptor gamma ELISA Kit
catalog :
MBS732320
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS732320
products type :
ELISA Kit
products full name :
Mouse Peroxisome Proliferators Activated Receptor gamma ELISA Kit
products short name :
Peroxisome Proliferators Activated Receptor gamma
products name syn :
Mouse Peroxisome Proliferators Activated Receptor g ELISA Kit
other names :
peroxisome proliferators-activated receptor gamma; peroxisome proliferators-activated receptor gamma
products gene name :
PPARgamma
products gene name syn :
PPARg
other gene names :
pparg
reactivity :
Mouse
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This PPARgamma ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse PPARgamma. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the Assay: PPARgamma ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PPARgamma antibody and an PPARgamma-HRP conjugate. The assay sample and buffer are incubated together with PPARgamma-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPARgamma concentration since PPARgamma from samples and PPARgamma-HRP conjugate compete for the anti-PPARgamma antibody binding site. Since the number of sites is limited, as more sites are occupied by PPARgamma from the sample, fewer sites are left to bind PPARgamma-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPARgamma concentration in each sample is interpolated from this standard curve.
ncbi gi num :
147904405
ncbi acc num :
NP_001091096.1
ncbi gb acc num :
NM_001097627.1
ncbi pathways :
PPAR Signaling Pathway (739863); PPAR Signaling Pathway (450)
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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