Human A Disintegrin And Metalloproteinase With Thrombospondin 1 ELISA Kit | MyBioSource MBS732313 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human A Disintegrin And Metalloproteinase With Thrombospondin 1 ELISA Kit

catalog :

MBS732313

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS732313

products type :

ELISA Kit

products full name :

Human A Disintegrin And Metalloproteinase With Thrombospondin 1 ELISA Kit

products short name :

A Disintegrin And Metalloproteinase With Thrombospondin 1

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 1 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 1; A disintegrin and metalloproteinase with thrombospondin motifs 1; METH-1; ADAM-TS1; ADAMTS-1; ADAM-TS 1; human metalloproteinase with thrombospondin type 1 motifs; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; METH-1

products gene name :

ADAMTS1

other gene names :

ADAMTS1; ADAMTS1; C3-C5; METH1; KIAA1346; METH1; ADAM-TS 1; ADAM-TS1; ADAMTS-1

uniprot entry name :

ATS1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of ADAMTS1. No significant cross-reactivity or interference between ADAMTS1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAMTS1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This ADAMTS1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAMTS1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cardiovascular

products description :

Principle of the assay: ADAMTS1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADAMTS1. Standards or samples are then added to the microtiter plate wells and ADAMTS1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADAMTS1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADAMTS1 are added to each well to “sandwich” the ADAMTS1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADAMTS1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

50845384

ncbi acc num :

NP_008919.3

ncbi gb acc num :

NM_006988.3

ncbi mol weight :

105,358 Da

ncbi pathways :

Degradation Of The Extracellular Matrix Pathway (576263); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)

ncbi summary :

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq, Jul 2008]

uniprot summary :

ADAMTS1: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. Protein type: Secreted; EC 3.4.24.-; Protease; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 21q21.2. Cellular Component: cytoplasm; basement membrane; cytoplasmic vesicle. Molecular Function: heparin binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity. Biological Process: integrin-mediated signaling pathway; negative regulation of cell proliferation; ovulation from ovarian follicle; proteolysis; kidney development

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage