ELISA/assay

Mouse Z-DNA binding protein ELISA Kit

MBS732283

48-Strip-Wells

470 USD

ELISA Kit

Mouse Z-DNA binding protein ELISA Kit

Z-DNA binding protein

Z-DNA-binding protein 1 isoform d; Z-DNA-binding protein 1; Z-DNA-binding protein 1; DNA-dependent activator of IRFs; DNA-dependent activator of IFN-regulatory factors; tumor stroma and activated macrophage protein DLM-1; DNA-dependent activator of interferon regulatory factors; Z-DNA binding protein 1; Tumor stroma and activated macrophage protein DLM-1

ZBP1

ZBP1; ZBP1; DAI; DLM1; DLM-1; C20orf183; C20orf183; DLM1

ZBP1_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of ZBP. No significant cross-reactivity or interference between ZBP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ZBP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 nmol/L.

Intended Uses: This ZBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ZBP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the assay: ZBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ZBP antibody and an ZBP-HRP conjugate. The assay sample and buffer are incubated together with ZBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZBP concentration since ZBP from samples and ZBP-HRP conjugate compete for the anti-ZBP antibody binding site. Since the number of sites is limited, as more sites are occupied by ZBP from the sample, fewer sites are left to bind ZBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZBP concentration in each sample is interpolated from this standard curve.

238550212

NP_001153891.1

NM_001160419.2

38,220 Da

Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); IRF3 Mediated Activation Of Type 1 IFN Pathway (576257); IRF3-mediated Induction Of Type I IFN Pathway (833819); Immune System Pathway (106386); Innate Immune System Pathway (106387); RIP-mediated NFkB Activation Via ZBP1 Pathway (576258); Regulation Of Innate Immune Responses To Cytosolic DNA Pathway (833823); STING Mediated Induction Of Host Immune Responses Pathway (685548)

This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

ZBP1: Participates in the detection by the host s innate immune system of DNA from viral, bacterial or even host origin. Plays a role in host defense against tumors and pathogens. Acts as a cytoplasmic DNA sensor which, when activated, induces the recruitment of TBK1 and IRF3 to its C-terminal region and activates the downstream interferon regulatory factor (IRF) and NF-kappa B transcription factors, leading to type-I interferon production. ZBP1-induced NF-kappaB activation probably involves the recruitment of the RHIM containing kinases RIPK1 and RIPK3. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; DNA-binding. Chromosomal Location of Human Ortholog: 20q13.31. Cellular Component: cytoplasm; nucleus; cytosol. Molecular Function: double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; left-handed Z-DNA binding; RNA binding. Biological Process: metabolic process; positive regulation of interferon type I production; innate immune response

48-Strip-Wells

470 USD

96-Strip-Wells

675