ELISA/assay

Human Retinoic Acid Early Transcript 1L ELISA Kit

MBS732277

96 Strip Wells

675 USD

ELISA Kit

Human Retinoic Acid Early Transcript 1L ELISA Kit

Retinoic Acid Early Transcript 1L

retinoic acid early transcript 1L protein; Retinoic acid early transcript 1L protein; retinoic acid early transcript 1L protein; UL16 binding protein 6; retinoic acid early transcript 1L

RAET1L

RAET1L; RAET1L; ULBP6

RET1L_HUMAN

Human

Store all reagents at 2-8 degree C.

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Signal Transduction

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This BG RAET1L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RAET1L. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY RAET1L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAET1L antibody and an RAET1L-HRP conjugate. The assay sample and buffer are incubated together with RAET1L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAET1L concentration since RAET1L from samples and RAET1L-HRP conjugate compete for the anti-RAET1L antibody binding site. Since the number of sites is limited, as more sites are occupied by RAET1L from the sample, fewer sites are left to bind RAET1L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAET1L concentration in each sample is interpolated from this standard curve.

126723295

NP_570970.2

NM_130900.2

27,509 Da

Natural Killer Cell Mediated Cytotoxicity Pathway 83079!!Natural Killer Cell Mediated Cytotoxicity Pathway 490

RAET1L belongs to the RAET1 family of major histocompatibility complex (MHC) class I-related genes, which are located within a 180-kb cluster on chromosome 6q24.2-q25.3. The REAT1 genes encode glycoproteins that contain extracellular alpha-1 and alpha-2 domains, but they lack the membrane proximal Ig-like alpha-3 domain. Most RAET1 glycoproteins are anchored to the membrane via glycosylphosphatidylinositol (GPI) linkage (Radosavljevic et al., 2002 [PubMed 11827464]).[supplied by OMIM, Mar 2008]

96 Strip Wells

675 USD