ELISA/assay

Mouse Lipolysaccharide binding protein ELISA Kit

MBS732251

48-Strip-Wells

470 USD

ELISA Kit

Mouse Lipolysaccharide binding protein ELISA Kit

Lipolysaccharide binding protein

lipopolysaccharide-binding protein; Lipopolysaccharide-binding protein; lipopolysaccharide-binding protein; LPS-binding protein; BPI fold containing family D, member 2; lipopolysaccharide binding protein

LBP

LBP; LBP; BPIFD2; LBP

LBP_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This LPS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse LPS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY LPS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LPS. Standards or samples are then added to the microtiter plate wells and LPS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LPS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LPS are added to each well to “sandwich” the LPS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LPS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPS concentration in each sample is interpolated from this standard curve.

31652249

NP_004130.2

NM_004139.4

53,384 Da

IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Innate Immune System Pathway (106387); NF-kappa B Signaling Pathway (634527); Regulation Of Toll-like Receptor Signaling Pathway (920955); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149); Toll Like Receptor 4 (TLR4) Cascade Pathway (106398); Toll-Like Receptors Cascades Pathway (106388); Toll-like Receptor Signaling Pathway (83076)

The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]

LBP: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 20q11.23. Cellular Component: extracellular space; cell surface; extracellular region. Molecular Function: protein binding; lipopolysaccharide binding; receptor binding. Biological Process: positive regulation of tumor necrosis factor biosynthetic process; detection of molecule of bacterial origin; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; positive regulation of tumor necrosis factor production; defense response to Gram-negative bacterium; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production; positive regulation of interleukin-8 production; lipopolysaccharide transport; defense response to Gram-positive bacterium; macrophage activation during immune response; lipopolysaccharide-mediated signaling pathway; acute-phase response; toll-like receptor signaling pathway; innate immune response; cellular defense response; opsonization; toll-like receptor 4 signaling pathway; positive regulation of macrophage activation

48-Strip-Wells

470 USD

96-Strip-Wells

675