Guinea pig Tissue Factor ELISA Kit | MyBioSource MBS732223 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Guinea pig Tissue Factor ELISA Kit

catalog :

MBS732223

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS732223

products type :

ELISA Kit

products full name :

Guinea pig Tissue Factor ELISA Kit

products short name :

Tissue Factor

other names :

tissue factor; Tissue factor; tissue factor; coagulation factor III (thromboplastin, tissue factor); Coagulation factor III; Thromboplastin; CD_antigen: CD142

products gene name :

other gene names :

F3; F3; TF; TFA; CD142; TF

uniprot entry name :

TF_HUMAN

reactivity :

Guinea Pig

specificity :

This assay has high sensitivity and excellent specificity for detection of TF. No significant cross-reactivity or interference between TF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TF and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This TF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig TF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cardiovascular

products description :

Principle of the assay: TF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TF antibody and an TF-HRP conjugate. The assay sample and buffer are incubated together with TF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TF concentration since TF from samples and TF-HRP conjugate compete for the anti-TF antibody binding site. Since the number of sites is limited, as more sites are occupied by TF from the sample, fewer sites are left to bind TF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TF concentration in each sample is interpolated from this standard curve.

ncbi gi num :

339506

ncbi acc num :

AAA61152.1

ncbi mol weight :

27,145 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Extrinsic Pathway (106058); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028)

ncbi summary :

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

uniprot summary :

F3: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Lung, placenta and pancreas. Belongs to the tissue factor family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1p22-p21. Cellular Component: extracellular matrix; extracellular space; cell surface; plasma membrane; integral to membrane. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protease binding; phospholipid binding. Biological Process: caspase activation; activation of blood coagulation via clotting cascade; positive regulation of protein kinase B signaling cascade; activation of plasma proteins during acute inflammatory response; positive regulation of angiogenesis; blood coagulation, extrinsic pathway; positive regulation of positive chemotaxis; cytokine and chemokine mediated signaling pathway; positive regulation of endothelial cell proliferation; blood coagulation; positive regulation of cell migration

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage