Mouse Beta Site APP Cleaving Enzyme 1 ELISA Kit | MyBioSource MBS732219 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Beta Site APP Cleaving Enzyme 1 ELISA Kit

catalog :

MBS732219

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS732219

products type :

ELISA Kit

products full name :

Mouse Beta Site APP Cleaving Enzyme 1 ELISA Kit

products short name :

Beta Site APP Cleaving Enzyme 1

other names :

Beta-site APP-cleaving enzyme 1; Beta-secretase 1; beta-secretase 1; asp 2; memapsin-2; APP beta-secretase; aspartyl protease 2; beta-site APP cleaving enzyme 1; beta-secretase 1 precursor variant 1; transmembrane aspartic proteinase Asp2; membrane-associated aspartic protease 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme; beta-site APP-cleaving enzyme 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2

products gene name :

bACE1

other gene names :

BACE1; BACE1; ASP2; BACE; HSPC104; BACE; KIAA1149; ASP2; Asp 2; Beta-site APP cleaving enzyme 1

uniprot entry name :

BACE1_HUMAN

reactivity :

Mouse

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This BACE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BACE1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the Assay: BACE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BACE1 antibody and an BACE1-HRP conjugate. The assay sample and buffer are incubated together with BACE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BACE1 concentration since BACE1 from samples and BACE1-HRP conjugate compete for the anti-BACE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BACE1 from the sample, fewer sites are left to bind BACE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BACE1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

41350854

ncbi acc num :

AAH65492.1

ncbi mol weight :

42,107 Da

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448)

ncbi summary :

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

uniprot summary :

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described. Protein type: Protease; Membrane protein, integral; EC 3.4.23.46. Chromosomal Location of Human Ortholog: 11q23.2-q23.3. Cellular Component: multivesicular body; Golgi apparatus; cell surface; cytoplasmic vesicle membrane; endoplasmic reticulum; integral to plasma membrane; axon; late endosome; plasma membrane; integral to membrane; trans-Golgi network; endosome. Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity. Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage