catalog number :
MBS732171
products type :
ELISA Kit
products full name :
Rabbit HSV Thymidine Kinase ELISA Kit
products short name :
HSV Thymidine Kinase
other names :
HSV thymidine kinase
products gene name :
HSV-TK
specificity :
This assay has high sensitivity and excellent specificity for detection of TK-HSV. No significant cross-reactivity or interference between TK-HSV and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TK-HSV and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This TK-HSV ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit TK-HSV. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Microbiology
products description :
. Principle of the assay: TK-HSV ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TK-HSV antibody and an TK-HSV-HRP conjugate. The assay sample and buffer are incubated together with TK-HSV-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TK-HSV concentration since TK-HSV from samples and TK-HSV-HRP conjugate compete for the anti-TK-HSV antibody binding site. Since the number of sites is limited, as more sites are occupied by TK-HSV from the sample, fewer sites are left to bind TK-HSV-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TK-HSV concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ADV71607.1