ELISA/assay

Human Augmenter of Liver Regeneration ELISA Kit

MBS732120

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Augmenter of Liver Regeneration ELISA Kit

Augmenter of Liver Regeneration

ALR; Histone-lysine N-methyltransferase 2D; histone-lysine N-methyltransferase 2D; ALL1-related protein; Kabuki make-up syndrome; Kabuki mental retardation syndrome; trinucleotide repeat containing 21; histone-lysine N-methyltransferase MLL2; myeloid/lymphoid or mixed-lineage leukemia 2; lysine (K)-specific methyltransferase 2D; ALL1-related protein; Myeloid/lymphoid or mixed-lineage leukemia protein 2

ALR

KMT2D; KMT2D; ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114; ALR; MLL2; MLL4; Lysine N-methyltransferase 2D

KMT2D_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of ALR. No significant cross-reactivity or interference between ALR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALR and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This ALR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ALR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: ALR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALR. Standards or samples are then added to the microtiter plate wells and ALR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALR present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ALR are added to each well to “sandwich” the ALR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALR concentration in each sample is interpolated from this standard curve.

2358287

AAC51735.1

593,677 Da

Disease Pathway (530764); Lysine Degradation Pathway (82956); Lysine Degradation Pathway (320); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1016209); Signal Transduction Pathway (477114); Signaling By WNT In Cancer Pathway (1016191); Signaling By Wnt Pathway (106510); TCF Dependent Signaling In Response To WNT Pathway (980475); XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway (1016210); Deactivation Of The Beta-catenin Transactivating Complex Pathway (980480)

The protein encoded by this gene is a histone methyltransferase that methylates the Lys-4 position of histone H3. The encoded protein is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the beta-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome. [provided by RefSeq, Oct 2010]

MLL4: Histone methyltransferase. Methylates Lys-4 of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. Defects in MLL2 are the cause of Kabuki syndrome type 1 (KABUK1). A congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nuclear receptor co-regulator; Methyltransferase; Transcription regulation; Methyltransferase, protein lysine; EC 2.1.1.43. Chromosomal Location of Human Ortholog: 12q13.12. Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus. Molecular Function: protein binding; DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific). Biological Process: oogenesis; oocyte growth; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to estrogen stimulus; chromatin silencing; positive regulation of cell proliferation; positive regulation of estrogen receptor signaling pathway; histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter. Disease: Kabuki Syndrome 1

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675