This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Toll Like Receptor4 ELISA Kit
catalog :
MBS732114
quantity :
48-Strip-Wells
price :
440 USD
product information
catalog number :
MBS732114
products type :
ELISA Kit
products full name :
Mouse Toll Like Receptor4 ELISA Kit
products short name :
[Toll Like Receptor4]
other names :
[TLR4, partial; TLR4; TLR4; Toll-like receptor 4]
products gene name :
[TLR4]
other gene names :
[TLR4]
uniprot entry name :
D0EL75_BUBBU
reactivity :
Mouse
specificity :
This assay has high sensitivity and excellent specificity for detection of TLR-4. No significant cross-reactivity or interference between TLR-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TLR-4 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 0.5-10ng/mL. Sensitivity: 0.1ng/mL
products categories :
Immunology
products description :
Intended Uses: This TLR-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TLR-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TLR-4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TLR-4. Standards or samples are then added to the microtiter plate wells and TLR-4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TLR-4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TLR-4 are added to each well to "sandwich" the TLR-4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TLR-4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR-4 concentration in each sample is interpolated from this standard curve.
ncbi gi num :
260401829
ncbi acc num :
ACX37356.1
ncbi mol weight :
6,356 Da
uniprot summary :
Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
size1 :
48-Strip-Wells
price1 :
440 USD
size2 :
96-Strip-Wells
price2 :
640
size3 :
5x96-Strip-Wells
price3 :
2895
size4 :
10x96-Strip-Wells
price4 :
5415
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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