ELISA/assay

Human Teratocarcinoma Derived Growth Factor 1 ELISA Kit

MBS732113

48-Strip-Wells-(Competitive)

440 USD

ELISA Kit

Human Teratocarcinoma Derived Growth Factor 1 ELISA Kit

[Teratocarcinoma Derived Growth Factor 1]

[teratocarcinoma-derived growth factor 1 isoform 2; Teratocarcinoma-derived growth factor 1; teratocarcinoma-derived growth factor 1; cripto-1 growth factor; epidermal growth factor-like cripto protein CR1; teratocarcinoma-derived growth factor 1; Cripto-1 growth factor; CRGF; Epidermal growth factor-like cripto protein CR1]

[TDGF1]

[TDGF1; TDGF1; CR; CRGF; CRIPTO; CRIPTO; CRGF]

TDGF1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of TCF4. No significant cross-reactivity or interference between TCF4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TCF4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This TCF4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TCF4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TCF4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TCF4 antibody and an TCF4-HRP conjugate. The assay sample and buffer are incubated together with TCF4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TCF4 concentration since TCF4 from samples and TCF4-HRP conjugate compete for the anti-TCF4 antibody binding site. Since the number of sites is limited, as more sites are occupied by TCF4 from the sample, fewer sites are left to bind TCF4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TCF4 concentration in each sample is interpolated from this standard curve.

292494883

NP_001167607.1

NM_001174136.1

21,169 Da

Developmental Biology Pathway (477129); Glypican 1 Network Pathway (138010); POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway (980456); Regulation Of Signaling By NODAL Pathway (477131); Signaling By NODAL Pathway (477130); Transcriptional Regulation Of Pluripotent Stem Cells Pathway (980455)

This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

TDGF1: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. Interacts with the activin type-1 receptor ACVR1B. Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. Expressed in breast and lung. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, peripheral; Cell cycle regulation; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: extrinsic to plasma membrane; Golgi apparatus; extracellular space; cell surface; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; nucleus; lipid raft. Molecular Function: protein binding; growth factor activity; receptor binding. Biological Process: somatic stem cell maintenance; activation of MAPK activity; heart development; regulation of signal transduction; gastrulation; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; positive regulation of fibroblast proliferation; mammary gland development; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; vasculogenesis; cell differentiation; epidermal growth factor receptor signaling pathway; in utero embryonic development; morphogenesis of a branching structure; positive regulation of peptidyl-tyrosine phosphorylation; embryonic development; peptidyl-serine phosphorylation; cell migration during sprouting angiogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of apoptosis; positive regulation of cell migration

48-Strip-Wells-(Competitive)

440 USD

48-Strip-Wells-(Sandwich)

440

96-Strip-Wells-(Competitive)

640

96-Strip-Wells-(Sandwich)

640

5x96-Strip-Wells-(Competitive)

2895

5x96-Strip-Wells-(Sandwich)

2895

10x96-Strip-Wells-(Competitive)

5415

10x96-Strip-Wells-(Sandwich)

5415