catalog number :
MBS732080
products type :
ELISA Kit
products full name :
Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA Kit
products short name :
Phosphorylation P38 Mitogen activated Protein Kinase
products gene name :
P P38MAPK
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Sensitivity: 1.0 ng/mL.
products categories :
Cell Biology
products description :
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This pMAPK-P38 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human pMAPK-P38. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY pMAPK-P38 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for pMAPK-P38. Standards or samples are then added to the microtiter plate wells and pMAPK-P38 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of pMAPK-P38 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for pMAPK-P38 are added to each well to “sandwich” the pMAPK-P38 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain pMAPK-P38 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The pMAPK-P38 concentration in each sample is interpolated from this standard curve.
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)