Human Fucosidase, Alpha L1, Tissue ELISA Kit | MyBioSource MBS732061 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Fucosidase, Alpha L1, Tissue ELISA Kit

catalog :

MBS732061

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS732061

products type :

ELISA Kit

products full name :

Human Fucosidase, Alpha L1, Tissue ELISA Kit

products short name :

Fucosidase, Alpha L1, Tissue

other names :

fucosidase, alpha-L- 1, tissue; Tissue alpha-L-fucosidase; tissue alpha-L-fucosidase; alpha-L-fucosidase 1; alpha-L-fucosidase I; alpha-L-fucoside fucohydrolase 1; fucosidase, alpha-L- 1, tissue; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; Alpha-L-fucosidase 1

products gene name :

FUCa1

other gene names :

FUCA1; FUCA1; FUCA; Nbla10230; Alpha-L-fucosidase 1

uniprot entry name :

FUCO_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of AFU. No significant cross-reactivity or interference between AFU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AFU and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This AFU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AFU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Signal Transduction

products description :

Principle of the assay: AFU ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AFU. Standards or samples are then added to the microtiter plate wells and AFU if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AFU present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AFU are added to each well to “sandwich” the AFU immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AFU and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFU concentration in each sample is interpolated from this standard curve.

ncbi gi num :

119615504

ncbi acc num :

EAW95098.1

ncbi mol weight :

53,689 Da

ncbi pathways :

Lysosome Pathway (99052); Lysosome Pathway (96865); Other Glycan Degradation Pathway (82976); Other Glycan Degradation Pathway (346)

ncbi summary :

The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]

uniprot summary :

FUCA1: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins. Defects in FUCA1 are the cause of fucosidosis (FUCA1D). FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. Belongs to the glycosyl hydrolase 29 family. Protein type: Hydrolase; EC 3.2.1.51; Glycan Metabolism - other glycan degradation. Chromosomal Location of Human Ortholog: 1p34. Cellular Component: lysosome; cytoplasm. Molecular Function: alpha-L-fucosidase activity; fucose binding. Biological Process: fucose metabolic process; glycosaminoglycan catabolic process; glycoside catabolic process. Disease: Fucosidosis

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage