ELISA/assay

Rat Soluble Fms-like Tyrosine 1 ELISA Kit

MBS732055

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Soluble Fms-like Tyrosine 1 ELISA Kit

Soluble Fms-like Tyrosine 1

soluble fms-like tyrosine kinase 1; Flt1 protein; vascular endothelial growth factor receptor 1; flt1a; receptor tyrosine kinase Flt1b; soluble fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); Flt1 protein; Soluble Fms-related tyrosine kinase 1/soluble vascular endothelial growth factor receptor 1 isoform sFlt1-11a; Soluble fms-like tyrosine kinase 1

sFLT1

flt1; flt1; flt1b; sFlt1; VEGFR1; sFlt1; sflt1

A4QNT7_DANRE

Rat

This assay has high sensitivity and excellent specificity for detection of sFLT-1. No significant cross-reactivity or interference between sFLT-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sFLT-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL

Intended Uses: This sFLT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat sFLT-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: sFLT-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for sFLT-1. Standards or samples are then added to the microtiter plate wells and sFLT-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of sFLT-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for sFLT-1 are added to each well to "sandwich" the sFLT-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain sFLT-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLT-1 concentration in each sample is interpolated from this standard curve.

324499394

ADY39752.1

53,549 Da

Adaptive Immune System Pathway (1004110); Constitutive PI3K/AKT Signaling In Cancer Pathway (1003436); Cytokine-cytokine Receptor Interaction Pathway (119260); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (1004203); DAP12 Signaling Pathway (1004204); Disease Pathway (1003331); Downstream Signal Transduction Pathway (1003616); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1004121); Downstream Signaling Of Activated FGFR Pathway (1003377)

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675