ELISA/assay

Human Catenin, beta ELISA Kit

MBS732036

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Catenin, beta ELISA Kit

Catenin, beta

Human Catenin, b ELISA Kit

catenin beta-1; Catenin beta-1; catenin beta-1; catenin (cadherin-associated protein), beta 1, 88kDa; Beta-catenin

CTNNb1

CTNNb1

CTNNB1; CTNNB1; CTNNB; MRD19; armadillo; CTNNB; OK/SW-cl.35; PRO2286

CTNB1_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

Intended Uses: This CTNNbeta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CTNNbeta. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This CTNNbeta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CTNNbeta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: CTNNbeta ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CTNNbeta. Standards or samples are then added to the microtiter plate wells and CTNNbeta if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CTNNbeta present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CTNNbeta are added to each well to "sandwich" the CTNNbeta immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CTNNbeta and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTNNbeta concentration in each sample is interpolated from this standard curve.

148233338

NP_001091679.1

NM_001098209.1

9,501 Da

AMER1 Mutants Destabilize The Destruction Complex Pathway (1016198); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1016193); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1016194); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1016197); AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway (1016195); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Adherens Junctions Interactions Pathway (119533); Adipogenesis Pathway (198832); Androgen Receptor Signaling Pathway (198806)

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009]

CTNNB1: a regulator of cell adhesion and a key downstream effector in the Wnt signaling pathway. Implicated early embryonic development and tumorigenesis. Phosphorylated and destabilized by CK1 and GSK-3beta. Stabilized cytoplasmic beta-catenin is a hallmark of a variety of cancers. Stabilized beta-catenin translocates to the nucleus, where it acts as a transcriptional activator of T-cell factor (TCF)-regulated genes. Interacts with the PDZ domain of TAX1BP3, inhibiting its transcriptional activity. Two alternatively spliced human isoforms have been described. Protein type: Oncoprotein; Nuclear receptor co-regulator; Actin-binding; Cell adhesion; Transcription factor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3p21. Cellular Component: centrosome; basolateral plasma membrane; fascia adherens; intercellular junction; cytosol; beta-catenin destruction complex; transcription factor complex; cell-cell adherens junction; membrane; lamellipodium; perinuclear region of cytoplasm; cytoplasm; synapse; dendritic shaft; lateral plasma membrane; spindle pole; focal adhesion; tight junction; catenin complex; cell cortex; Z disc; nucleoplasm; adherens junction; apical part of cell; microvillus membrane; plasma membrane; nucleus; cell junction. Molecular Function: protein C-terminus binding; transcription coactivator activity; transcription factor binding; protein phosphatase binding; ionotropic glutamate receptor binding; signal transducer activity; protein binding; enzyme binding; androgen receptor binding; cadherin binding; double-stranded DNA binding; protein complex binding; estrogen receptor binding; nitric-oxide synthase binding; SMAD binding; kinase binding; transcription factor activity; nuclear hormone receptor binding; alpha-catenin binding. Biological Process: regulation of myelination; regulation of centriole-centriole cohesion; positive regulation of apoptosis; protein heterooligomerization; positive regulation of transcription, DNA-dependent; regulation of fibroblast proliferation; cell maturation; negative regulation of chondrocyte differentiation; T cell differentiation in the thymus; positive regulation of fibroblast growth factor receptor signaling pathway; osteoclast differentiation; Wnt receptor signaling pathway through beta-catenin; cell-cell adhesion; positive regulation of endothelial cell differentiation; regulation of cell fate specification; embryonic foregut morphogenesis; positive regulation of mesenchymal cell proliferation; male genitalia development; synapse organization and biogenesis; ectoderm development; cell adhesion; bone resorption; response to drug; positive regulation of neuroblast proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; regulation of smooth muscle cell proliferation; hair cell differentiation; negative regulation of protein sumoylation; genitalia morphogenesis; patterning of blood vessels; muscle cell differentiation; midgut development; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; oocyte development; embryonic forelimb morphogenesis; negative regulation of osteoclast differentiation; glial cell fate determination; endodermal cell fate commitment; apoptosis; cell-matrix adhesion; neuron migration; dorsal/ventral axis specification; cell fate specification; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; embryonic hindlimb morphogenesis; response to estradiol stimulus; negative regulation of cell proliferation; central nervous system vasculogenesis; positive regulation of MAPKKK cascade; pancreas development; positive regulation of interferon type I production; fallopian tube development; proximal/distal pattern formation; layer formation in the cerebral cortex; cell structure disassembly during apoptosis; negative regulation of mitotic cell cycle, embryonic; Wnt receptor signaling pathway; hair follicle morphogenesis; thymus development; in utero embryonic development; embryonic axis specification; stem cell maintenance; regulation of T cell proliferation; neural plate development; synaptic vesicle transport; gastrulation with mouth forming second; liver development; regulation of angiogenesis; odontogenesis of dentine-containing teeth; negative regulation of oligodendrocyte differentiation; myoblast differentiation; positive regulation of osteoblast differentiation; Schwann cell proliferation; response to cadmium ion; ureteric bud branching; response to cytokine stimulus; androgen receptor signaling pathway; epithelial to mesenchymal transition; embryonic heart tube development; innate immune response; positive regulation of muscle cell differentiation; lens morphogenesis in camera-type eye; anterior/posterior axis specification. Disease: Pilomatrixoma; Mental Retardation, Autosomal Dominant 19; Ovarian Cancer; Colorectal Cancer; Hepatocellular Carcinoma

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675