Human A Disintegrin Like and Metalloproteinase with Thrombospondin type 1 motif 7 ELISA Kit | MyBioSource MBS732016 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human A Disintegrin Like and Metalloproteinase with Thrombospondin type 1 motif 7 ELISA Kit

catalog :

MBS732016

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS732016

products type :

ELISA Kit

products full name :

Human A Disintegrin Like and Metalloproteinase with Thrombospondin type 1 motif 7 ELISA Kit

products short name :

A Disintegrin Like and Metalloproteinase with Thrombospondin type 1 motif 7

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 7 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 7; A disintegrin and metalloproteinase with thrombospondin motifs 7; COMPase; a disintegrin and metalloprotease with thrombospondin motifs-7 preproprotein; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 7; ADAM metallopeptidase with thrombospondin type 1 motif, 7; COMPase

products gene name :

ADAMTS7

other gene names :

ADAMTS7; ADAMTS7; ADAM-TS7; ADAMTS-7; ADAM-TS 7; ADAM-TS 7; ADAM-TS7; ADAMTS-7

uniprot entry name :

ATS7_HUMAN

reactivity :

Human

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

products categories :

Signal Transduction

products description :

Intended Uses: This ADAMTS7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAMTS7. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ADAMTS7 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADAMTS7. Standards or samples are then added to the microtiter plate wells and ADAMTS7 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADAMTS7 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADAMTS7 are added to each well to "sandwich" the ADAMTS7 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADAMTS7 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS7 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

38683827

ncbi acc num :

NP_055087.2

ncbi gb acc num :

NM_014272.3

ncbi mol weight :

184,095 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)

ncbi summary :

The protein encoded by this gene is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two C-terminal TS motifs. [provided by RefSeq, Jul 2008]

uniprot summary :

ADAMTS7: Metalloprotease that may play a role in the degradation of COMP. Protein type: EC 3.4.24.-; Motility/polarity/chemotaxis; Secreted, signal peptide; Protease; Cell surface; Secreted. Chromosomal Location of Human Ortholog: 15q24.2. Cellular Component: proteinaceous extracellular matrix; cell surface; endoplasmic reticulum lumen. Molecular Function: protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; proteolysis involved in cellular protein catabolic process; negative regulation of chondrocyte differentiation; post-translational protein modification

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage