catalog number :
MBS732006
products type :
ELISA Kit
products full name :
Human beta-Hydroxybutyric Acid ELISA Kit
products short name :
beta-Hydroxybutyric Acid
products name syn :
Human beta-Hydroxybutyric Acid ELISA Kit; beta-Hydroxybutyric Acid; b-Hydroxybutyric Acid; -Hydroxybutyric Acid; beta-Hydroxybutyric Acid (Human)
products gene name :
beta-OHB
products gene name syn :
b-OHB; -OHB
specificity :
This assay has high sensitivity and excellent specificity for detection of betaHA. No significant cross-reactivity or interference between betaHA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between betaHA and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 umol/L.
other info2 :
Intended Uses: This betaHA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human betaHA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Human ELISA Kit
products description :
Principle of the Assay: betaHA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-betaHA antibody and an betaHA-HRP conjugate. The assay sample and buffer are incubated together with betaHA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the betaHA concentration since betaHA from samples and betaHA-HRP conjugate compete for the anti-betaHA antibody binding site. Since the number of sites is limited, as more sites are occupied by betaHA from the sample, fewer sites are left to bind betaHA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The betaHA concentration in each sample is interpolated from this standard curve.