ELISA/assay

Mouse Soluble protein-100B ELISA Kit

MBS732004

48-Strip-Wells

470 USD

ELISA Kit

Mouse Soluble protein-100B ELISA Kit

Soluble protein-100B

Mouse Soluble protein-100B ELISA Kit; Soluble protein-100B; Soluble protein-100B (Mouse)

S100 calcium binding protein, beta (neural), isoform CRA_a; Protein S100-B; protein S100-B; OTTHUMP00000115888; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural); S100 calcium binding protein B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B

S-100B

S100B; S100B; NEF; S100; S100-B; S100beta

S100B_HUMAN

Mouse

Sensitivity: The sensitivity in this assay is1.0 pg/mL. Specificity: This assay has high sensitivity and excellent specificity for detection of S-100B. No significant cross-reactivity or interference between S-100B and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

Mouse ELISA Kit

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma. Intended Uses: This S-100B ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of S-100B in the sample, this S-100B ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus S-100B concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of S-100B utilizes a multiclonal anti-S-100B antibody and an S-100B-HRP conjugate. The assay sample and buffer are incubated together with S-100B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S-100B concentration since S-100B from samples and S-100B-HRP conjugate compete for the anti-S-100B antibody binding site. Since the number of sites is limited, as more sites are occupied by S-100B from the sample, fewer sites are left to bind S-100B-HRP conjugate. Standards of known S-100B concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of S-100B. The S-100B concentration in each sample is interpolated from this standard curve.

119629675

P04271

10,713 Da

Activated TLR4 Signalling Pathway (106400); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Immune System Pathway (106386); Innate Immunity Signaling Pathway (106387); MyD88 Cascade Initiated On Plasma Membrane Pathway (205107); MyD88 Dependent Cascade Initiated On Endosome Pathway (187081); MyD88-independent Cascade Initiated On Plasma Membrane Pathway (106401); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (106390); NFkB And MAP Kinases Activation Mediated By TLR4 Signaling Repertoire Pathway (187089); RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways (187097)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq]

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: ruffle; extracellular space; cell soma; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus. Molecular Function: identical protein binding; RAGE receptor binding; protein binding; protein homodimerization activity; zinc ion binding; calcium ion binding; calcium-dependent protein binding; tau protein binding. Biological Process: central nervous system development; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; response to glucocorticoid stimulus; memory; regulation of cell shape; cell proliferation; learning and/or memory; astrocyte differentiation; axonogenesis; positive regulation of cell proliferation; response to methylmercury; innate immune response; regulation of neuronal synaptic plasticity

48-Strip-Wells

470 USD

96-Strip-Wells

675