Human Alpha Smooth Muscle Actin ELISA Kit | MyBioSource MBS731995 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Alpha Smooth Muscle Actin ELISA Kit

catalog :

MBS731995

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS731995

products type :

ELISA Kit

products full name :

Human Alpha Smooth Muscle Actin ELISA Kit

products short name :

Alpha Smooth Muscle Actin

other names :

actin, aortic smooth muscle; Actin, aortic smooth muscle; actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein; actin, alpha 2, smooth muscle, aorta; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

products gene name :

AlphaSMA

other gene names :

ACTA2; ACTA2; AAT6; ACTSA; MYMY5; ACTSA; ACTVS; GIG46

uniprot entry name :

ACTA_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of SMAalpha. No significant cross-reactivity or interference between SMAalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SMAalpha and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL

products categories :

Signal Transduction

products description :

Intended Uses: This SMAalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SMAalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: SMAalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SMAalpha. Standards or samples are then added to the microtiter plate wells and SMAalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SMAalpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SMAalpha are added to each well to "sandwich" the SMAalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SMAalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SMAalpha concentration in each sample is interpolated from this standard curve.

ncbi gi num :

213688375

ncbi acc num :

NP_001135417.1

ncbi gb acc num :

NM_001141945.1

ncbi mol weight :

42,009 Da

ncbi pathways :

Muscle Contraction Pathway (106261); Myometrial Relaxation And Contraction Pathways (198759); Smooth Muscle Contraction Pathway (161025); Striated Muscle Contraction Pathway (198903); Vascular Smooth Muscle Contraction Pathway (96530); Vascular Smooth Muscle Contraction Pathway (96866)

ncbi summary :

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

uniprot summary :

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family. Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Contractile. Chromosomal Location of Human Ortholog: 10q23.3. Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton. Molecular Function: protein kinase binding; ATP binding. Biological Process: muscle contraction; regulation of blood pressure; response to virus; vascular smooth muscle contraction. Disease: Multisystemic Smooth Muscle Dysfunction Syndrome; Moyamoya Disease 5; Aortic Aneurysm, Familial Thoracic 6

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage