ELISA/assay

Human Filamin B, beta ELISA Kit

MBS731914

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Filamin B, beta ELISA Kit

Filamin B, beta

Human Filamin B, b ELISA Kit

filamin-B isoform 4; Filamin-B; filamin-B; filamin-3; beta-filamin; ABP-280 homolog; filamin homolog 1; thyroid autoantigen; actin binding protein 278; actin-binding-like protein; Larsen syndrome 1 (autosomal dominant); filamin B, beta; ABP-278; ABP-280 homolog; Actin-binding-like protein; Beta-filamin; Filamin homolog 1; Fh1; Filamin-3; Thyroid autoantigen; Truncated actin-binding protein; Truncated ABP

FLNB

FLNB

FLNB; FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280; FLN1L; FLN3; TABP; TAP; FLN-B; Fh1; Truncated ABP

FLNB_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of FLNB. No significant cross-reactivity or interference between FLNB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FLNB and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This FLNB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FLNB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: FLNB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FLNB. Standards or samples are then added to the microtiter plate wells and FLNB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FLNB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FLNB are added to each well to "sandwich" the FLNB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FLNB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FLNB concentration in each sample is interpolated from this standard curve.

256222415

NP_001157791.1

NM_001164319.1

276,939 Da

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); ISG15 Antiviral Mechanism Pathway (530761); Immune System Pathway (106386); Interferon Signaling Pathway (187103); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Proteoglycans In Cancer Pathway (782000)

This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]

FLNB: a ubiquitous cytoskeletal protein that links actin filaments to membrane glycoproteins. Six alternatively spliced isoforms have been described. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoforms 1 and 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells, retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord and platelets. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer s disease. Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart. Protein type: Actin-binding; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3p14.3. Cellular Component: focal adhesion; cytoplasm; integral to membrane; stress fiber; plasma membrane; cell cortex; Z disc; cytosol; actin cytoskeleton. Molecular Function: identical protein binding; protein binding; actin binding. Biological Process: skeletal muscle development; cytoskeletal anchoring; cytokine and chemokine mediated signaling pathway; cell differentiation; actin cytoskeleton organization and biogenesis; signal transduction. Disease: Larsen Syndrome; Boomerang Dysplasia; Atelosteogenesis, Type I; Spondylocarpotarsal Synostosis Syndrome; Atelosteogenesis, Type Iii

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675