Human Epididymis Protein 4 ELISA Kit | MyBioSource MBS731817 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Epididymis Protein 4 ELISA Kit

catalog :

MBS731817

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS731817

products type :

ELISA Kit

products full name :

Human Epididymis Protein 4 ELISA Kit

products short name :

Epididymis Protein 4

other names :

WAP four-disulfide core domain protein 2; WAP four-disulfide core domain protein 2; WAP four-disulfide core domain protein 2; epididymal protein 4; epididymal secretory protein E4; putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; major epididymis-specific protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; WAP four-disulfide core domain 2; Epididymal secretory protein E4; Major epididymis-specific protein E4; Putative protease inhibitor WAP5

products gene name :

HE 4

other gene names :

WFDC2; WFDC2; HE4; WAP5; EDDM4; dJ461P17.6; HE4; WAP5

uniprot entry name :

WFDC2_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of EP-4. No significant cross-reactivity or interference between EP-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EP-4 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pmol/ml.

other info2 :

Intended Uses: This EP-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human EP-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: EP-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EP-4 antibody and an EP-4-HRP conjugate. The assay sample and buffer are incubated together with EP-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EP-4 concentration since EP-4 from samples and EP-4-HRP conjugate compete for the anti-EP-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by EP-4 from the sample, fewer sites are left to bind EP-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EP-4 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

56699495

ncbi acc num :

NP_006094.3

ncbi gb acc num :

NM_006103.3

ncbi mol weight :

11,043 Da

ncbi summary :

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

uniprot summary :

WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: extracellular space. Molecular Function: serine-type endopeptidase inhibitor activity; aspartic-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; cysteine protease inhibitor activity. Biological Process: spermatogenesis; proteolysis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage