ELISA/assay

Human Glycoprotein V, Platelet ELISA Kit

MBS731812

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Glycoprotein V, Platelet ELISA Kit

Glycoprotein V, Platelet

gp5; Gp5; gp5; Gp5

GP5

5; 5; KVP40.0335

Q6WHG9_BPKVM

Human

This assay has high sensitivity and excellent specificity for detection of PCL5. No significant cross-reactivity or interference between PCL5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PCL5 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This PCL5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PCL5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

. Principle of the assay: PCL5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PCL5. Standards or samples are then added to the microtiter plate wells and PCL5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PCL5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PCL5 are added to each well to "sandwich" the PCL5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PCL5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCL5 concentration in each sample is interpolated from this standard curve.

34419568

NP_899581.1

NC_005083.2

46,083 Da

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675