ELISA/assay

Rat Low density lipoprotein receptor ELISA Kit

MBS731723

48-Strip-Wells

470 USD

ELISA Kit

Rat Low density lipoprotein receptor ELISA Kit

Low density lipoprotein receptor

low density lipoprotein receptor; Low-density lipoprotein receptor; low-density lipoprotein receptor; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3; low density lipoprotein receptor

LDLR

LDLR; LDLR; FH; FHC; LDLCQ2; LDL receptor

LDLR_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This LDLR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat LDLR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the Assay: LDLR ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LDLR. Standards or samples are then added to the microtiter plate wells and LDLR if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LDLR present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for LDLR are added to each well to "sandwich" the LDLR immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LDLR and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LDLR concentration in each sample is interpolated from this standard curve.

307121

AAA56833.1

82,255 Da

Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Chylomicron-mediated Lipid Transport Pathway (106157); DNA Damage Response (only ATM Dependent) Pathway (198827); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907)

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, misc.; Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: Golgi apparatus; cell surface; membrane; lysosome; integral to plasma membrane; early endosome; late endosome; plasma membrane; coated pit; endosome membrane; receptor complex; external side of plasma membrane. Molecular Function: very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; protein binding; low-density lipoprotein binding; calcium ion binding; clathrin heavy chain binding; glycoprotein binding. Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; phototransduction, visible light; lipoprotein catabolic process; cholesterol homeostasis; cholesterol transport; viral reproduction; cholesterol absorption; phospholipid transport; endocytosis; lipoprotein metabolic process; lipid metabolic process; retinoid metabolic process. Disease: Hypercholesterolemia, Familial

48-Strip-Wells

470 USD

96-Strip-Wells

675