ELISA/assay

Mouse Anti-Complement 1q Antibody ELISA Kit

MBS731701

48-Strip-Wells

470 USD

ELISA Kit

Mouse Anti-Complement 1q Antibody ELISA Kit

Anti-Complement 1q Antibody

complement C1q tumor necrosis factor-related protein 5; Complement C1q tumor necrosis factor-related protein 5; complement C1q tumor necrosis factor-related protein 5; myonectin; C1q TNF-alpha-related protein 5; C1q and tumor necrosis factor related protein 5

C1q

C1QTNF5; C1QTNF5; CTRP5; CTRP5; UNQ303/PRO344

C1QT5_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This C1Q-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C1Q-Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY C1Q-Ab ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with Complement 1Q antigen. Standards or samples are then added to the microtiter plate wells and C1Q-Ab if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of C1Q-Ab present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated Complement 1Q antigen is added to each well to “sandwich” the C1Q-Ab immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain C1Q-Ab and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1Q-Ab concentration in each sample is interpolated from this standard curve.

14149712

NP_056460.1

NM_015645.4

25,298 Da

This gene encodes a member of a family of proteins that function as components of basement membranes and may play a role in cell adhesion. Mutations in this gene have been associated with late-onset retinal degeneration. The protein may be encoded by either a bicistronic transcript including sequence from the upstream membrane frizzled-related protein gene (MFRP), or by a monocistronic transcript expressed from an internal promoter. [provided by RefSeq, Jun 2013]

C1QTNF5: Defects in C1QTNF5 are a cause of late-onset retinal degeneration (LORD). LORD is an autosomal dominant disorder characterized by onset in the fifth to sixth decade with night blindness and punctate yellow-white deposits in the retinal fundus, progressing to severe central and peripheral degeneration, with choroidal neovascularization and chorioretinal atrophy. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: collagen. Disease: Late-onset Retinal Degeneration

48-Strip-Wells

470 USD

96-Strip-Wells

675