ELISA/assay

Rat Proteoglycan 4 ELISA Kit

MBS731653

48-Strip-Wells

470 USD

ELISA Kit

Rat Proteoglycan 4 ELISA Kit

Proteoglycan 4

proteoglycan 4, partial; Proteoglycan 4; Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycanCleaved into the following chain:Proteoglycan 4 C-terminal part

PRG4

PRG4; MSF; SZP

PRG4_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of PRG-4. No significant cross-reactivity or interference between PRG-4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRG-4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This PRG-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat PRG-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: PRG-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRG-4 antibody and an PRG-4-HRP conjugate. The assay sample and buffer are incubated together with PRG-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRG-4 concentration since PRG-4 from samples and PRG-4-HRP conjugate compete for the anti-PRG-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by PRG-4 from the sample, fewer sites are left to bind PRG-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRG-4 concentration in each sample is interpolated from this standard curve.

50345548

AAT74746.1

146,468 Da

PRG4: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Defects in PRG4 are the cause of camptodactyly- arthropathy-coxa vara-pericarditis syndrome (CACP); also known as Jacobs syndrome. CACP is an autosomal recessive disorder. Individuals with CACP have normal appearing joints at birth but with advancing age develop joint failure associated with noninflammatory synoviocyte hyperplasia and subintimal fibrosis of the synovial capsule. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q25-q31. Cellular Component: extracellular space. Molecular Function: scavenger receptor activity; polysaccharide binding. Biological Process: receptor-mediated endocytosis; cell proliferation; negative regulation of interleukin-6 biosynthetic process; immune response; regulation of cell proliferation. Disease: Camptodactyly-arthropathy-coxa Vara-pericarditis Syndrome

48-Strip-Wells

470 USD

96-Strip-Wells

675