ELISA/assay

Guinea pig Aldehyde dehydrogenase family 1 member A3 ELISA Kit

MBS731605

48-Strip-Wells

470 USD

ELISA Kit

Guinea pig Aldehyde dehydrogenase family 1 member A3 ELISA Kit

Aldehyde dehydrogenase family 1 member A3

aldehyde dehydrogenase family 1 member A3 isoform 1; Aldehyde dehydrogenase family 1 member A3; aldehyde dehydrogenase family 1 member A3; aldehyde dehydrogenase 6; acetaldehyde dehydrogenase 6; retinaldehyde dehydrogenase 3; aldehyde dehydrogenase 1 family, member A3; Aldehyde dehydrogenase 6; Retinaldehyde dehydrogenase 3; RALDH-3; RalDH3

ALDH1A3

ALDH1A3; ALDH1A3; ALDH6; MCOP8; RALDH3; ALDH1A6; ALDH6; RALDH-3; RalDH3

AL1A3_HUMAN

Guinea Pig

Store all reagents at 2-8 degree C.

Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma. Intended Uses: This APN ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of APN in the sample, this APN ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus APN concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of APN utilizes a multiclonal anti-APN antibody and an APN-HRP conjugate. The assay sample and buffer are incubated together with APN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APN concentration since APN from samples and APN-HRP conjugate compete for the anti-APN antibody binding site. Since the number of sites is limited, as more sites are occupied by APN from the sample, fewer sites are left to bind APN-HRP conjugate. Standards of known APN concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of APN. The APN concentration in each sample is interpolated from this standard curve.

153266822

NP_000684.2

NM_000693.3

56,108 Da

Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glycolysis / Gluconeogenesis Pathway (82926); Glycolysis / Gluconeogenesis Pathway (287); Histidine Metabolism Pathway (82958); Histidine Metabolism Pathway (324); Metabolic Pathways (132956); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031)

This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

ALDH1A3: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system. Belongs to the aldehyde dehydrogenase family. Protein type: Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - histidine; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - phenylalanine; EC 1.2.1.5; Xenobiotic Metabolism - metabolism by cytochrome P450. Chromosomal Location of Human Ortholog: 15q26.3. Cellular Component: cytoplasm; cytosol. Molecular Function: aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; aldehyde dehydrogenase [NAD(P)+] activity; retinal dehydrogenase activity. Biological Process: inner ear morphogenesis; retinal metabolic process; retinol metabolic process; embryonic eye morphogenesis; positive regulation of apoptosis; righting reflex; olfactory pit development; optic cup morphogenesis involved in camera-type eye development; retinoic acid metabolic process; locomotory behavior; neuromuscular process controlling balance; nucleus accumbens development. Disease: Microphthalmia, Isolated 8

48-Strip-Wells

470 USD

96-Strip-Wells

675