ELISA/assay

Human Phospho Glycogen Synthase Kinase 3beta ELISA Kit

MBS731570

48-Strip-Wells

470 USD

ELISA Kit

Human Phospho Glycogen Synthase Kinase 3beta ELISA Kit

Phospho Glycogen Synthase Kinase 3beta

Human Phospho Glycogen Synthase Kinase 3b ELISA Kit

podocin isoform 1; Podocin; podocin; nephrosis 2, idiopathic, steroid-resistant (podocin)

P GSK3beta

P GSK3b

NPHS2; NPHS2; PDCN; SRN1

PODO_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of PDCN. No significant cross-reactivity or interference between PDCN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PDCN and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This PDCN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PDCN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PDCN ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PDCN. Standards or samples are then added to the microtiter plate wells and PDCN if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PDCN present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PDCN are added to each well to "sandwich" the PDCN immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PDCN and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDCN concentration in each sample is interpolated from this standard curve.

7657615

NP_055440.1

NM_014625.3

34,421 Da

Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway (138072)

This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q25.2. Cellular Component: protein complex; integral to plasma membrane; endoplasmic reticulum; plasma membrane; intercellular junction; lipid raft. Molecular Function: protein binding. Biological Process: actin cytoskeleton reorganization; excretion. Disease: Nephrotic Syndrome, Type 2

48-Strip-Wells

470 USD

96-Strip-Wells

675