ELISA/assay

Human Metallothionein ELISA Kit

MBS731548

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Metallothionein ELISA Kit

Metallothionein

metallothionein; Metallothionein; metallothionein; MT; metallothionein 2A; metallothionein 4

MT

MT4; MT; MT2A; MT

MT_CHICK

Human

This assay has high sensitivity and excellent specificity for detection of MT. No significant cross-reactivity or interference between MT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MT and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This MT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Microbiology

. Principle of the assay: MT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MT. Standards or samples are then added to the microtiter plate wells and MT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MT are added to each well to "sandwich" the MT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT concentration in each sample is interpolated from this standard curve.

46048711

NP_990606.1

NM_205275.1

6,462 Da

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675