catalog number :
MBS731540
products type :
ELISA Kit
products full name :
Rat Angiotensin (1-7) ELISA Kit
products short name :
Angiotensin (1-7)
other names :
Angiotensin, partial; Angiotensin
products gene name :
ANG (1-7)
uniprot entry name :
ANGT_THETS
specificity :
This assay has high sensitivity and excellent specificity for detection of ANG-(1-7). No significant cross-reactivity or interference between ANG-(1-7) and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANG-(1-7) and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Cardiovascular
products description :
Intended Uses: This ANG-(1-7) ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ANG-(1-7). This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay ANG-(1-7) ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANG-(1-7) antibody and an ANG-(1-7)-HRP conjugate. The assay sample and buffer are incubated together with ANG-(1-7)-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANG-(1-7) concentration since ANG-(1-7) from samples and ANG-(1-7)-HRP conjugate compete for the anti-ANG-(1-7) antibody binding site. Since the number of sites is limited, as more sites are occupied by ANG-(1-7) from the sample, fewer sites are left to bind ANG-(1-7)-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANG-(1-7) concentration in each sample is interpolated from this standard curve.