ELISA/assay

Human Carbonic Anhydrase I ELISA Kit

MBS731524

48-Strip-Wells

470 USD

ELISA Kit

Human Carbonic Anhydrase I ELISA Kit

Carbonic Anhydrase I

CA1 protein; Carbonic anhydrase 1; carbonic anhydrase 1; carbonic anhydrase B; carbonate dehydratase I; epididymis secretory protein Li 11; carbonic anhydrase I; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I

CA1

CA1; CA1; CAB; CA-I; Car1; HEL-S-11; CAB; CA-I

CAH1_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 IU/mL.

Intended Uses: This CA-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CA-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CA-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CA-1. Standards or samples are then added to the microtiter plate wells and CA-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CA-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CA-1 are added to each well to "sandwich" the CA-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CA-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA-1 concentration in each sample is interpolated from this standard curve.

20380766

AAH27890.1

28,870 Da

C-MYB Transcription Factor Network Pathway (138073); Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway (645347); Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway (645348); Metabolism Pathway (477135); Nitrogen Metabolism Pathway (83025); Nitrogen Metabolism Pathway (416); O2/CO2 Exchange In Erythrocytes Pathway (645346); Reversible Hydration Of Carbon Dioxide Pathway (645352)

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family. Protein type: Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen. Chromosomal Location of Human Ortholog: 8q21.2. Cellular Component: cytosol. Molecular Function: carbonate dehydratase activity; zinc ion binding. Biological Process: bicarbonate transport; one-carbon compound metabolic process

48-Strip-Wells

470 USD

96-Strip-Wells

675