Guinea pig Interferon alpha ELISA Kit | MyBioSource MBS731511 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Guinea pig Interferon alpha ELISA Kit

catalog :

MBS731511

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS731511

products type :

ELISA Kit

products full name :

Guinea pig Interferon alpha ELISA Kit

products short name :

Interferon alpha

products name syn :

Guinea pig Interferon a ELISA Kit

other names :

interferon-alpha; Interferon alpha-8; interferon alpha-8; leIF B; IFN-alpha-8; interferon alpha-B; interferon alpha-B'; interferon alpha-B2; interferon alpha type 201; interferon, alpha 8; Interferon alpha-B; LeIF B; Interferon alpha-B2

products gene name :

IFN-alpha

products gene name syn :

IFN-a

other gene names :

IFNA8; IFNA8; IFN-alphaB; IFN-alpha-8; LeIF B

uniprot entry name :

IFNA8_HUMAN

reactivity :

Guinea Pig

specificity :

This assay has high sensitivity and excellent specificity for detection of IFN?. No significant cross-reactivity or interference between IFN? and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFN? and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This IFN? ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig IFN?. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cancer

products description :

Principle of the assay: IFN? ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IFN?. Standards or samples are then added to the microtiter plate wells and IFN? if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IFN? present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IFN? are added to each well to “sandwich” the IFN? immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN? and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFN? concentration in each sample is interpolated from this standard curve.

ncbi gi num :

306902

ncbi acc num :

AAA52716.1

ncbi mol weight :

21,989 Da

ncbi pathways :

Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Hepatitis B Pathway (694606)

uniprot summary :

IFNA8: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family. Protein type: Secreted; Secreted, signal peptide; Cytokine. Chromosomal Location of Human Ortholog: 9p22. Cellular Component: extracellular space; extracellular region. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; natural killer cell activation during immune response; regulation of MHC class I biosynthetic process; innate immune response; blood coagulation; defense response to virus; humoral immune response; T cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage