ELISA/assay

Mouse Dopamine Transporter ELISA Kit

MBS731509

48-Strip-Wells

440 USD

ELISA Kit

Mouse Dopamine Transporter ELISA Kit

[Dopamine Transporter]

[Dat; Dat; D-alanine aminotransferase Dat; hypothetical protein; Dat]

[DAT]

[dat; dat; HRM2_21590]

C0QDJ3_DESAH

Mouse

This assay has high sensitivity and excellent specificity for detection of DAT. No significant cross-reactivity or interference between DAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DAT and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Detection Range: 0.5-10ng/mL. Sensitivity: 0.1ng/mL

Neurobiology

Intended Uses This DAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DAT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: DAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DAT antibody and an DAT-HRP conjugate. The assay sample and buffer are incubated together with DAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DAT concentration since DAT from samples and DAT-HRP conjugate compete for the anti-DAT antibody binding site. Since the number of sites is limited, as more sites are occupied by DAT from the sample, fewer sites are left to bind DAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DAT concentration in each sample is interpolated from this standard curve.

223691974

ACN15257.1

31,763 Da

Arginine And Proline Metabolism Pathway (92440); Arginine And Proline Metabolism Pathway (323); D-Alanine Metabolism Pathway (92452); D-Alanine Metabolism Pathway (342); D-Arginine And D-ornithine Metabolism Pathway (92451); D-Arginine And D-ornithine Metabolism Pathway (341); Lysine Degradation Pathway (92439); Lysine Degradation Pathway (320); Metabolic Pathways (132880); Phenylalanine Metabolism Pathway (92443)

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415