catalog number :
MBS731499
products type :
ELISA Kit
products full name :
Mouse Insulin Autoantibody ELISA Kit
products short name :
Insulin Autoantibody
specificity :
This assay has high sensitivity and excellent specificity for detection of IAA. No significant cross-reactivity or interference between IAA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IAA and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Immunology
products description :
Intended Uses: This IAA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IAA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay IAA ELISA kit applies the competitive enzyme immunoassay technique utilizing Insulin antigen and an IAA-HRP conjugate. The assay sample and buffer are incubated together with IAA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IAA concentration since IAA from samples and IAA-HRP conjugate compete for the Insulin antigen binding site. Since the number of sites is limited, as more sites are occupied by IAA from the sample, fewer sites are left to bind IAA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IAA concentration in each sample is interpolated from this standard curve.