product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Chymotrypsin ELISA Kit
catalog :
MBS731485
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
product information
catalog number :
MBS731485
products type :
ELISA Kit
products full name :
Rat Chymotrypsin ELISA Kit
products short name :
Chymotrypsin
other names :
chymotrypsin; Chymotrypsin
uniprot entry name :
CTR_PHACE
reactivity :
Rat
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
products description :
Intended Uses: This Chymotrypsin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat Chymotrypsin. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: Chymotrypsin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Chymotrypsin antibody and an Chymotrypsin-HRP conjugate. The assay sample and buffer are incubated together with Chymotrypsin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Chymotrypsin concentration since Chymotrypsin from samples and Chymotrypsin-HRP conjugate compete for the anti-Chymotrypsin antibody binding site. Since the number of sites is limited, as more sites are occupied by Chymotrypsin from the sample, fewer sites are left to bind Chymotrypsin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Chymotrypsin concentration in each sample is interpolated from this standard curve.
ncbi gi num :
4210802
ncbi acc num :
CAA76928.1
uniprot acc num :
O97398
ncbi mol weight :
29,868 Da
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!