catalog number :
MBS731484
products type :
ELISA Kit
products full name :
Mouse Free Prostate Specific Antigen ELISA Kit
products short name :
Free Prostate Specific Antigen
products gene name :
FPSA
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: is 1.0 pg/mL.
other info2 :
Intended Uses: This fPSA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse fPSA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Neurobiology
products description :
Principle of the Assay: fPSA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-fPSA antibody and an fPSA-HRP conjugate. The assay sample and buffer are incubated together with fPSA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the fPSA concentration since fPSA from samples and fPSA-HRP conjugate compete for the anti-fPSA antibody binding site. Since the number of sites is limited, as more sites are occupied by fPSA from the sample, fewer sites are left to bind fPSA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The fPSA concentration in each sample is interpolated from this standard curve.