Mouse P-selectin glycoprotein ligand 1 ELISA Kit | MyBioSource MBS731419 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse P-selectin glycoprotein ligand 1 ELISA Kit

catalog :

MBS731419

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS731419

products type :

ELISA Kit

products full name :

Mouse P-selectin glycoprotein ligand 1 ELISA Kit

products short name :

P-selectin glycoprotein ligand 1

other names :

P-selectin glycoprotein ligand 1 isoform 2; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen; selectin P ligand; Selectin P ligand; CD_antigen: CD162

products gene name :

PSGL-1

other gene names :

SELPLG; SELPLG; CLA; CD162; PSGL1; PSGL-1; PSGL-1

uniprot entry name :

SELPL_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of PSGL-1. No significant cross-reactivity or interference between PSGL-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PSGL-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This PSGL-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PSGL-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the Assay: PSGL-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PSGL-1 antibody and an PSGL-1-HRP conjugate. The assay sample and buffer are incubated together with PSGL-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PSGL-1 concentration since PSGL-1 from samples and PSGL-1-HRP conjugate compete for the anti-PSGL-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PSGL-1 from the sample, fewer sites are left to bind PSGL-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSGL-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

331284236

ncbi acc num :

NP_002997.2

ncbi gb acc num :

NM_003006.4

ncbi mol weight :

44,648 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); Staphylococcus Aureus Infection Pathway (172846); Staphylococcus Aureus Infection Pathway (171867); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

uniprot summary :

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12q24. Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane. Molecular Function: protein binding; receptor binding. Biological Process: cell adhesion; blood coagulation; leukocyte tethering or rolling; leukocyte adhesive activation; leukocyte migration

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage