ELISA/assay

Rat Anti-bullous pemphigoid 180 antibody ELISA Kit

MBS731382

48-Strip-Wells

440 USD

ELISA Kit

Rat Anti-bullous pemphigoid 180 antibody ELISA Kit

[Anti-bullous pemphigoid 180 antibody]

[180 kDa bullous pemphigoid antigen 2/type XVII collagen; Collagen alpha-1(XVII) chain; collagen alpha-1(XVII) chain; collagen alpha-1(XVII) chain; alpha 1 type XVII collagen; type XVII collagen alpha-1; collagen XVII, alpha-1 polypeptide; 180 kDa bullous pemphigoid antigen 2; bullous pemphigoid antigen 2 (180kD); bA16H23.2 (collagen, type XVII, alpha 1 (BP180)); collagen, type XVII, alpha 1; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2Cleaved into the following 2 chains:120 kDa linear IgA disease antigenAlternative name(s):120 kDa linear IgA dermatosis antigen; Linear IgA disease antigen 1; LAD-1]

[BP180-Ab]

[COL17A1; COL17A1; BP180; BPA-2; BPAG2; LAD-1; BA16H23.2; BP180; BPAG2; LAD-1; 97 kDa LAD antigen; 97-LAD; LABD97]

COHA1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of anti-ascaris lumbricoides antibody (IgM). No significant cross-reactivity or interference between anti-ascaris lumbricoides antibody (IgM) and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between anti-ascaris lumbricoides antibody (IgM) and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ug/mL.

Signal Transduction

Intended Uses: This anti-ascaris lumbricoides antibody (IgM) ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human anti-ascaris lumbricoides antibody (IgM). This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: anti-ascaris lumbricoides antibody (IgM) ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-anti-ascaris lumbricoides antibody (IgM) antibody and an anti-ascaris lumbricoides antibody (IgM)-HRP conjugate. The assay sample and buffer are incubated together with anti-ascaris lumbricoides antibody (IgM)-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti-ascaris lumbricoides antibody (IgM) concentration since anti-ascaris lumbricoides antibody (IgM) from samples and anti-ascaris lumbricoides antibody (IgM)-HRP conjugate compete for the anti-anti-ascaris lumbricoides antibody (IgM) antibody binding site. Since the number of sites is limited, as more sites are occupied by anti-ascaris lumbricoides antibody (IgM) from the sample, fewer sites are left to bind anti-ascaris lumbricoides antibody (IgM)-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti-ascaris lumbricoides antibody (IgM) concentration in each sample is interpolated from this standard curve.

1877435

AAB51499.1

142,344 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Degradation Pathway (730309); Collagen Formation Pathway (645288); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]

COL17A1: May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. Defects in COL17A1 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). GABEB is a non- lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Extracellular matrix; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 10q24.3. Cellular Component: collagen; hemidesmosome; integral to plasma membrane; endoplasmic reticulum lumen; extracellular region; plasma membrane; basement membrane; intercellular junction. Molecular Function: protein binding. Biological Process: extracellular matrix disassembly; collagen catabolic process; epidermis development; extracellular matrix organization and biogenesis; hemidesmosome assembly; cell-matrix adhesion. Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415